Abstract:
The need for new donors of resistance to aggressive race Ug99 of Puccinia graminis Pers. f. sp. tritici Eriks. et Henn has arisen because the pest approaches the territory of Russian Federation. Identification of seventeen known Sr genes was performed with use of molecular markers in six sources of resistance to stem rust race Ug99. They included five common wheat lines and Donskaya Polukarlikovaya cv. The investigated accessions carried genetic material of alien species (Aegilops speltoides, Aе. triuncialis, Secale cereale). We used molecular markers associated with effective genes to race Ug99: Sr2, Sr22, Sr24, Sr25, Sr26, Sr32, Sr35, Sr36, Sr39, Sr40, Sr44, Sr47, and with genes that were ineffective but protected wheat cultivars against local pathogen populations: Sr9a, Sr15, Sr17, Sr19 and Sr31. On the grounds of the analysis of molecular markers the presence of two to seven known Sr genes was postulated in analyzed wheat lines :Sr2, Sr36, Sr39, Sr40, Sr44, Sr47 and Sr15 in line 113/00i-4; Sr22, Sr32, Sr44, Sr9a, Sr17 and Sr19 in line 119/4-06rw; Sr24, Sr36, Sr40, Sr47, Sr15, Sr17 and Sr31 in line GT 96/90; Sr22, Sr44, Sr32 and Sr15 in line 9/00w; Sr22 and Sr44 in line 141/97w. Genes Sr32 and Sr44 effective against Ug99 and ineffective genes Sr9a, Sr17 and Sr19 were identified in Donskaya Polukarlikovaya cv. The accessions studied may be recommended as donors of resistance to race Ug99 in wheat breeding programs.
About The Authors:
O. A. Baranova. All-Russia Institute for Plant Protection (FSBSI VIZR), Saint-Petersburg, Pushkin, Russia, Russian Federation
A. V. Anisimova. All-Russia Institute for Plant Protection (FSBSI VIZR), Saint-Petersburg, Pushkin, Russia, Russian Federation
I. V. Iordanskaya. Moscow Agricultural Research Institute «Nemchinovka», Moscow Oblast, Russia, Russian Federation
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63. Rahmatov M., Andrsson S., Gustavsson L., Wanyera R., Steffenson B., Johansson E. Analysis of adult plant and seedling stem rust resistance in wheat-alien introgression lines. Disease Risk and Food Security: Proc. of the 13th Int. Cereal Rusts and Powdery Mildew Conf. 28 Aug.-1 Sept. 2012, Beijing. 2012.
64. Shamanin V.P., Morgunov A.I., Manes J., Zelensky Y.I., Chursin, A.S., Levshunov M.A., Pototskaya. I.V., Likhenko I.E., Manko T.A., Karakoz I.I., Tabachenko A.V., Petukhovsky S.L. Breeding and genetic estimation of spring bread wheat populations of the Siberian shuttle breeding nursery of CIMMYT. Vavilovskii zhurnal genetiki i selektsii — Vavilov Journal of Genetics and Breeding.2012;16(1): 21-32.
65. Sinyak E.V., Volkova G.V., Nadykta V.D. Characteristics of the population Puccinia graminis f. sp. tritici virulence in the North Caucasus region of Russia. Doklady RASHN — Russian Agricultural Sciences. 2013;6:27-30.
66. Singh R.P., Hodson D.P., Jin Y., Huerta-Espino J., Kinyua M.G., Wanyera R., Njau P., Ward R.W. Current status, likely migration and strategies to mitigate the threat to wheat production from race Ug99 (TTKS) of stem rust pathogen. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources. 2006;1(054). DOI: 10.1079/PAVSNNR20061054
67. Singh R.P., Hodson D.P., Huerta-Espino J., Jin Y., Njau P., Wanyera R., Herrera-Foessel S.A., Ward R.W. Will stem rust destroy the world’s wheat crop? Advanced Agronomy. 2008;98:271-309. DOI: 10.1016/S0065-2113(08)00
68. Stakman E.C., Stewart D.M., Loegering W.Q. Identification of physiologic races of Puccinia graminis var. tritici. U.S. Dep. Agric. Agric. Res. Serv. E-617. U.S. Gov. Print. Office, Washington, DC, 1962.
69. Tsilo T.J., Jin Y., Anderson J.A. Diagnostic microsatellite markers for detection of stem rustresistance gene Sr36 in diverse genetic backgrounds of wheat. Crop Sci. 2008;48:253-261. DOI:10.2135/cropsci2007.04.0204
70. Volkova G.V., Shumilov Y.V., Sinyak E.V., Vaganova O.F., Danilova A. V. Effective resistance genes of wheat and barley to rust pathogens and their identification in promising cultivar accessions. Mat. Mezhdunar. nauch.-prak. konferencii “Biologicheskaja zashhita rastenij — osnova stabilizacii agrojekosistem” [Abstr. of the International Scientific-Practical Conference “Biological plant protection as the basis of ecosystem stabilization”]. Krasnodar, 2014.
71. William H.M., Trethowan R., Crosby-Galvan E.M. Wheat breeding assisted by markers: CIMMYT’s experience. Euphytica. 2007;157: 307-319. DOI: 10.1007/s10681-007-9405-7
72. Wu S., Pumphrey M., Bai G. Molecular mapping of stem-rust-resistance gene Sr40 in wheat. Crop Sci. 2009;49:1681-1686. DOI: 10.2135/cropsci2008.11.0666
73. Xu S.S., Klindworth D., Niu Z.-X., Zhang Q.-J., Chao S.-M., Friesen T., Jin Y., Zhong S.-B., Faris J., Cai X.-W., Larkin P. Introgression of alien genes for resistance to Ug99 stem rust in wheat. Disease Risk and Food Security: Proc. 13th Int. Cereal Rusts and Powdery Mildew Conf. 28 Aug.-1 Sept. 2012, Beijing. 2012.
74. Yu L.X., Liu S., Anderson J.A., Singh R.P., Jin Y., Dubcovsky J., Brown-Guidera G., Bhavani S., Morgounov A., He Z., Huerta-Espino J., Sorrells M.E. Haplotype diversity of stem rust resistance loci in uncharacterized wheat lines. Mol. Breeding. 2010;26:667-680. DOI: 10.1007/s11032-010-9403-7
75. Zheng Q., Klindworth D., Niu Z.-X., Rouse M., Yu J., Friesen T., Li Z.-Z., Xu S. Stem rust resistance in Thinopyrum species. Disease Risk and Food Security. Proc. 13th Int. Cereal Rusts and Powdery Mildew Conf. 28 Aug.-1 Sept. 2012, Beijing. 2012.
