Abstract:
Grey wheatgrass Agropyron glaucum (Desf. ex DC) Roem. & Schult is a valuable source of genes
for resistance to diseases, frost resistance, and salt tolerance. An unstable 76-chromosomal amphidiploid combining genomes A and B of common wheat variety Avrora, six chromosomes of genome D of the same variety, and a full set of Ag. glaucum (2n = 42) chromosomes was used as an intermediate to transfer the genetic material from the wild donor to the said wheat variety. A large set of wheat introgression lines differing in a variety of morphobiological characters was developed.
For effective employment of the developed lines in breeding, cytological and molecular-genetical analyses of the lines were conducted, and their pest resistance and grain technological properties were evaluated. We report the investigation of 25 common wheat introgression lines with genetic material from Ag. glaucum, not studied hitherto. All lines but D43 formed 21 bivalents in МI meiosis. In lines D3, D21, and D23, the genetic material of Ag. glaucum was present as a translocation segment. Lines D7, D43, and D49 carried substituted chromosomes and, presumably, translocations. One pair of wheat chromosomes was substituted in 18 lines. For the identification of translocations and substituted chromosomes, microsatellite analysis was done with markers specific to D genome chromosomes. The introgression touched all D genome chromosomes except 3D and 4D. The lines under the study differed in protein and gluten contents, gluten quality, and bread-making quality. Study of gliadin spectra revealed changes in the gliadin formula in 7 of 12 lines with reference to the recipient Avrora variety. Thus, the results obtained point to genetic diversity of investigated introgression lines and their value for common wheat breeding.
About The Authors:
R. O. Davoyan. SSI Krasnodar Research Institute of Agriculture, Krasnodar, Russia, Russian Federation
E. R. Davoyan. SSI Krasnodar Research Institute of Agriculture, Krasnodar, Russia, Russian Federation
Y. S. Zubanova. SSI Krasnodar Research Institute of Agriculture, Krasnodar, Russia, Russian Federation
References:
1. Aksel’rud D.V., Rybalka A.I. Original’nye agrotekhnologicheskie svoĭstva liniĭ ozimoĭ myagkoĭ pshenitsy na osnove chuzherodnykh introgressiĭ ot dikikh i kul’turnykh sorodicheĭ. Puti povysheniya i stabilizatsii proizvodstva vysokokachestvennogo zerna: Sb. dokl. Mezhdunar. nauch.-prakt. konf. Krasnodar, 2002:34-37.
2. Vakar B.A. Tsitologiya pshenichno-pyreĭnykh gibridov. Omsk: Omskoe oblastnoe izd-vo, 1935.
3. Davoyan R.O., Bebyakina I.V., Bessarab K.S. Poluchenie i kharakteristika chuzherodno-zameshchennykh liniĭ ozimoĭ myagkoĭ pshenitsy Avrora s khromosomami Agropyron glaucum. Evolyutsiya nauchnykh tekhnologiĭ v rastenievodstve: Sb. nauch. tr., posvyashch. 90-letiyu KNIISKh im. P.P. Luk’yanenko. Krasnodar, 2004; 3:3-9.
4. Davoyan R.O., Bebyakina I.V., Kekalo N.Yu. Identifikatsiya khromosom pyreya sizogo (Agropiron glaucum) u zameshchennykh liniĭ sorta myagkoĭ pshenitsy Avrora. Nauka Kubani. 2005;4: 104-107.
5. Davoyan R.O., Bebyakina I.V., Davoyan O.R., Zinchenko A.N., Davoyan E.R., Kravchenko A.M., Zubanova Yu.S. Sinteticheskie formy kak osnova dlya sokhraneniya i ispol’zovaniya genofonda dikikh sorodicheĭ myagkoĭ pshenitsy. Vavilovskiĭ zhurnal genetiki i selektsii. 2012;16(1):44-51.
6. Davoyan O.R. Izuchenie introgressivnykh liniĭ Triticum aestivum L. dlya ispol’zovaniya v selektsii myagkoĭ pshenitsy: Avtoref. dis. … kand. biol. nauk. Krasnodar, 2012.
7. Zhirov E.G., Ternovskaya T.K. Genomnaya inzheneriya u pshenitsy. Vestn. s.-kh. nauki. 1984;10:58-66.
8. Laĭkova L.I., Belan I.A., Badaeva E.D., Rosseeva L.P., Shepelev S.S. Sozdanie i izuchenie sorta yarovoĭ myagkoĭ pshenitsy «Pamyati Maĭstrenko» s introgressieĭ geneticheskogo materiala ot sinteticheskogo geksaploida Triticum timopheevii Zhuk. × Aegilops tauchii Coss. Genetika. 2013;49(1):103-112.
9. Metody selektsii i otsenki ustoĭchivosti pshenitsy i yachmenya k boleznyam v stranakh-chlenakh SEV. Praga, 1988.
10. Metodika Gosudarstvennogo sortoispytaniya sel’skokhozyaĭstvennykh kul’tur. M., 1988.
11. Peresypkin V.F. Bolezni zernovykh kul’tur. M.: Kolos, 1979: 122-124.
12. Pshenichnikova T.M., Ermakova M.F., Popova R.K. Tekhnologicheskie kachestva zerna i muki myagkoĭ pshenitsy v liniyakh s mezhsortovym zameshcheniem khromosom 1 i 6 gomeologichnykh grupp. S.-kh. biologiya. 2006;1:57-62.
13. Timonova E.M., Leonova I.N., Belan I.A., Rosseeva L.P., Salina E.A. Vliyanie otdel’nykh uchastkov khromosom Triticum timopheevii na formirovanie ustoĭchivosti k boleznyam i kolichestvennye priznaki myagkoĭ pshenitsy. Vavilovskiĭ zhurnal genetiki i selektsii. 2012;16(1):142-159.
14. Sozinov A.A., Poperelya F.A. Metodika vertikal’nogo diskovogo elektroforeza v krakhmal’nom gele i geneticheskiĭ printsip klassifikatsii gliadinov. Odessa, 1978.
15. Khizhnyak V.A. Formoobrazovanie u pshenichno-pyreĭnykh gibridov. Izd. AN SSSR. 1938:597-626.
16. Tsitsin N.V. Chto daet skreshchivanie pshenitsy s pyreem. M.: Sel’khozizdat, 1937.
17. Bochev B. The genus Aegilops — possibilities and perspectives of utilization the breeding of high quality wheat cultivar. Proc. of 7th World Cereal Genet. and Breed. Congr. Prague, 1983:237-242.
18. Brevis J.C., Chicaiza O., Khan I.A., Jackson L., Morris C.F., Dubcovsky J. Agronomic and quality evaluation of common wheat near-isogenic lines carrying the leaf rust resistance gene LR 47. Crop Sci. 2008;48:1441-1451.
19. Dysk P.L., Fiebe B. Evaluation of leaf rust resistance from wheat chromosomal translocation lines. Crop Sci. 1993;33:687-690.
20. Gasner G., Straib U.W. Weitere Untersuchungen uber die Spezialisierung sverhaltnissedes Gelbrostes Puccinia glumarum (Schm.) Erikss. u. Henn. Arb. Boil. Reichsanstalt. 1934;21:121-145.
21. Friebe B., Zeller F.J., Mukai Y., Forster B.R., Bartos P., McIntosh R.N. Characterization of rust-resistant wheatAgropyron intermedium derivatives by C-banding, in situ hybridization and isozyme analysis. Theor. Appl. Genet. 1992;83:775-782.
22. Friebe B., Jiang J., Raupp W.J., McIntosh R.A., Gill B.S. Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica. 1996;91:59-87.
23. Jauhar P.P., Peterson T.S. Thinopyrum and Lophopyrum as a sources of genes for wheat improvement. Cereal Res. Commun. 1996;24: 15-21.
24. Knott D.R. The effect of transfers of alien genes for leaf rust resistance on the agronomic and quality characteristics of wheat. Euphytica. 1989;44:65-72.
25. Li H., Wang X. Thinopyrum ponticum and Th. intermedium: the promising source of resistance to fungal and viral diseases of wheat. J. Genet. Genom. 2009;36:557-565.
26. Mains E.B., Jakson H.S. Physiologic specialization in leaf rust of wheat, Puccinia triticina Erikss. Phytopatology. 1926;16:89-120.
27. Mansur L.M., Qualset C.O., Kasarda D.D. Effects of «Cheyenne» chromosomes on milling and baking quality in «Chinese Spring» wheat in relation to glutenin and gliadin storage proteins. Crop Sci. 1990;30:593-602.
28. McGuire P.E., Dvorak J. High salt-tolerance potential in wheat grasses. Crop Sci. 1981;21(5):497-500.
29. McIntosh R.A., Devos K.M., Dubcovsky J., Rogers W.J., Morris C.F., Appels R., Anderson O.D. Catalogue of gene symbols for wheat: 2005 supplement. Ann. Wheat Newslet. 2005;51:272-285.
30. McIntosh R.A., Dubcovsky J., Rogers W.J., Morris C., Appels R., Xia X. Catalogue of gene symbols for wheat: 2009 supplement. Ann. Wheat Newslet. 2009;55:256-278.
31. Payne P.I., Holt L.M., Lawrence G.J. The genetics of gliadinis and glutenins, the major storage proteins of the wheat endosperm. Plant Foods Human Nutr. 1982;31:229-241.
32. Pestsova E., Ganal M.W., Roder M.S. Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome. 2000;43:689-697.
33. Worland A.J., Law C.N., Hollins T.W., Kabner R.M.D., Guira A. Location of a gene for resistance to eyesot (Pseudocercosporella herpotrichoides) on chromosome 7D of bread wheat. Plant Breeding. 1988;101(1):43-51.
34. Zeller F.J., Fuchs E. Cytologie und Krankheitsresistenz einer 1A/1R-und mehrerer 1B/1R-Weizen-Roggen-Translokatiossorten. Z. Pflanzenzucht. 1983;90(4):285-296.
35. Zeven A.C., Waninge J. The degree of similarly or backross lines of Triticum aestivum cultivars Manitou and Neepawa with Aegilops speltoides accessions as donor. Euphytica. 1986;35:677-685.
