Raise of short-stemmed vaviloid branched spike lines and their cytogenetics. A. J. Aliyeva, S. P. Mehdiyeva, R. K. Kerimova

Abstract:

Hybridization between hexaploid species of wheat (Triticum L.) and triticale (Ч Triticosecale Wittm.)
is used widely in genetic research and breeding when studying mutual introgressions of genetic material to original species. As far as the hexaploid triticale obtained in our Institute possessed a strong potential of morphogenesis, we decided to use it in crosses with bread wheat. During the process of morphogenesis in hybrid population of the second and third generations derived from the crosses between hexaploid triticale with different bread wheat cultivars (Opal and Chinese Spring) and variety (Triticum aestivum var. velutinum), plants with wheat, rye, intermediate, and triticale-like spike types were noted. Starting from the third generation, constant short-stemmed vaviloid branched spike plants were isolated among populations under study. Despite instability and numerous meiotic aberrations observed in the first generation of plants, including branched-spike ones, the subsequent progeny demonstrated meiotic and morphological stability. The chromosome composition of one of the lines (378/3SD) was characterized by fluorescence in situ hybridization (FISH) and genome in situ hybridization (GISH), and it was identified as a substitution line. Chromosomal identification of such lines will allow their use in genetic studies concerning specified traits, in particular, the vaviloid branched spike.

About The Authors:

A. J. Aliyeva. Genetic Resources Institute of ANAS, Baku, Azerbaijan, Russian Federation

S. P. Mehdiyeva. Genetic Resources Institute of ANAS, Baku, Azerbaijan, Russian Federation

R. K. Kerimova. Baku State Medical University, Baku, Azerbaijan, Russian Federation

References:

1. Alieva A.Dzh. Istochnik novogo tipa vetvistokolososti u tverdykh pshenits. Dokl. RASKhN. 2009;3:10-11.

2. Alieva A.Dzh., Aminov N.Kh. Vliyanie genoma D pshenitsy na proyavlenie priznaka novogo tipa vetvistokolososti v gibridnykh populyatsiyakh linii 171ACS. Genetika. 2013;49(11):1284-1291.

3. Gorin A.P., Dunin M.S., Konovalov Yu.B. Praktikum po selektsii i semenovodstvu polevykh kul’tur. M.: Kolos, 1968.

4. Dorofeev V.F. Spontannye mutatsii kak faktor formoobrazovaniya pshenitsy. Vestnik s.-kh. nauki. 1968;7:16-26.

5. Kurkiev K.U. Nasledovanie vysoty rasteniya u geksaploidnykh form tritikale s R/D zameshcheniem. Genetika. 2008;44(9): 1238-1245.

6. Luk’yanenko P.P., Kostin V.V. Novye formy tverdoi pshenitsy. Dokl. VASKhNIL. 1970;6:2-3.

7. Motsnyi I.I., Fait V.I., Blagodarova E.M. Identifikatsiya i kharakteristika 1R(1V) zameshchennykh linii myagkoi pshenitsy. Tsitologiya i genetika. 2009;43(3):26-35.

8. Pausheva Z.P. Praktikum po tsitologii rastenii. M.: Agropromizdat, 1988.

9. Silkova O.G., Shchapova A.I., Shumnyi V.K. Peredacha geneticheskogo materiala rzhi v genom myagkoi pshenitsy metodom mezhgenomnogo zameshcheniya khromosom. Informatsionnyi vestnik VOGiS. 2008;12(4):654-661.

10. Tumanyan M.G. Genofond pshenits Armenii. Tr. Arm. SKhI. 1935;1.

11. Filatenko A.A. Mezhvidovaya gibridizatsiya v rode Triticum L.: Avtoref. dis. … kand. biol. nauk. L.: VIR, 1968.

12. Flyaksberger K.A. Pshenitsa. Kul’turnaya flora SSSR. M.; L.: Gosizdat kolkh. i sovkh. lit-ry, 1935.

13. Tsitsin N.V. Teoriya i praktika otdalennoi gibridizatsii. AN SSSR. Gl. bot. sad. M.: Nauka, 1981.

14. Alkhimova A.G., Heslop-Harrison J.S., Shchapova A.I., Vershinin A.V. Rye chromosome variability in wheat-rye addition and substitution lines. Chromosome Res. 1999;7:205-212. DOI:10.1023/a:1009299300018

15. Bedbrook J.R., Jones J., O’Dell M., Thompson R.J., Flavell R.B. A molecular description of telomeric heterochromatin in Secale species. Cell. 1980;19:545-560.

16. Bento M., Gustafson P., Viegas W., Silva M. Genome merger: from sequence rearrangements in triticale to their elimination in wheatrye addition lines. Theor. Appl. Genet. 2010;121:489-497. DOI: 10.1007/s00122-010-1325-6

17. Carvalho A., Martín A., Heslop-Harrison J.S., Guedes-Pinto H., Lima-Brito J. Identification of the spontaneous 7BS/7RL intergenomic translocation in one F1 multigeneric hybrid from the Triticeae tribe. Plant Breeding. 2009;128:105-108. DOI: 10.1111/j.1439-0523.2008.01519.x

18. Gerlach W.L., Bedbrook J.L. Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucl. Acids Res. 1979;7:1869-1885.

19. Ji J., Wang Zh., Sun J., Li J., Zhang X., Wang D., Zhang A. Identification of new T1BL.1RS translocation lines derived from wheat (Triticum aestivum L. cultivar «Xiaoyan No. 6») and rye hybridization. Acta Physiol. Plant. 2008;30:689-695. DOI: 10.1007/s11738-008-0167-1

20. Jiang J., Friebe B., Gill B.S. Chromosome painting of «Amigo» wheat. Theor. Appl. Genet. 1994;8:811-813.

21. Lei M.P., Li G.R., Zhang S., Liu C., Yang Z. Molecular cytogenetic characterization of a new wheat Secale africanum 2Ra(2D) substitution line for resistance to stripe rust. J. Genet. 2011;90(2): 283-287.

22. Linc G., Friebe B.R., Kynast R.G., Molnár-Láng M., Köszegi B., Sutka J., Gill B.S. Molecular cytogenetic analysis of Aegilops cylindrica Host. Genome. 1999;42:497-503.

23. Mac Key J. Species relationship in Triticum. Proc. 2nd Intern. Wheat Genet. Symp.,1963. Hereditas Suppl., 1966;2:237-275.

24. Molnár-Láng M., Cseh A., Szakács E., Molnár I. Development of a wheat genotype combining the recessive crossability alleles kr1kr1kr2kr2 and the 1BL.1RS translocation, for the rapid enrichment of 1RS with new allelic variation. Theor. Appl. Genet. 2010;120(8):1535-1545. DOI: 10.1007/s00122-010-1274-0

25. Molnár-Láng M., Linc G., Friebe B.R., Sutka J. Detection of wheat-barley translocations by genomic in situ hybridization in derivatives of hybrids multiplied in vitro. Euphytica. 2000;112: 117-123.

26. Nagaki K., Tsujimoto H., Isono K., Sasakuma T. Molecular characterization of a tandem repeat, Afa family, and its distribution among Triticeae. Genome. 1995;38:479-486.

27. Reader S.M., Abbo S., Purdie K.A., King I.P., Miller T.E. Direct labelling of plant chromosomes by rapid in situ hybridization. Trends Genet. 1994;10:265-266.

28. Szakács E., Molnár-Láng M. Development and molecular cytogenetic identification of new winter wheat — winter barley («Martonvásári 9 kr1» — «Igri») disomic addition lines. Genome. 2007;50(1): 43-50. DOI:10.1139/g06-134.

29. Szakács É., Molnár-Láng M. Molecular cytogenetic evaluation of chromosome instability in Triticum aestivum-Secale cereale disomic addition lines. J. Appl. Genet. 2010;51:149-152. DOI: 10.1007/bf03195723

30. Zhou J., Zhang H., Yang Z., Li G., Hu L., Lei M., Liu C., Zhang J., Ren Z. Characterization of a new T2DS.2DL-?R translocation triticale ZH-1 with multiple resistances to diseases. Genet. Resour. Crop Ev. 2012;59:1161-1168. DOI: 10.1007/s10722-011-9751-0

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