Effects of growth factors during in vitro culture of mouse and rat embryos. E. Yu. Brusentsev, T. N. Igonina, I. N. Rozhkova, D. S. Ragaeva, S. Ya. Amstislavsky


In vitro culture of preimplantation embryos of ICR, HT1AN/Icgn, HT1AC/Icgn and C57BL/6J-Ay mouse strains as well as in OXYS/Icgn rat strain in media containing granulocyte-macrophage colony stimulating factor (GM-CSF) or epidermal growth factor (EGF) has been studied. Both mouse and rat embryos were first frozen in a programmable freezer after a standard protocol using a mixture of glycerol and sucrose as cryoprotectants, thawed and cultured in vitro in R1ECM (rat one-cell embryo culture medium) for 24 hours (mice) and 72 hours (rats). For the in vitro culture experiments with these growth factors, 8-cell frozen-thawed mouse embryos and 2–4-cell frozen-thawed rat embryos were used. Supplementation of the culture medium with GM-CSF improved the rate of embryonic development in HT1AC/Icgn and C57BL/6J-Ay strain mice, while EGF had no effect. The reverse was true of the rats. Supplementation of the culture medium with EGF increased the percentage of deve­loping blastocysts in OXYS/Icgn rat strain, while GM-CSF had no effect. Co-culture of four-cell embryos of HT1AN/ Icgn strain mice with more advanced embryonic stages (morulas) of a different strain ICR led to the facilitation preimplantation embryo development. Experimental results presented here reveal the species-specific effects of growth factors on mouse and rat embryos and indicate that co-culture of different stages of embryo development have stimulatory effects on earlier stages.

About The Authors:

E. Yu. Brusentsev. Institute of Cytology and Genetics SB RAS, Russian Federation, Novosibirsk

T. N. Igonina. Institute of Cytology and Genetics SB RAS, Russian Federation, Novosibirsk

I. N. Rozhkova. Institute of Cytology and Genetics SB RAS, Russian Federation, Novosibirsk

D. S. Ragaeva. Institute of Cytology and Genetics SB RAS, Russian Federation, Novosibirsk

S. Ya. Amstislavsky. Institute of Cytology and Genetics SB RAS, Russian Federation, Novosibirsk


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