Category Archives: Tom 19-4
A comparison of different cryoprotectant solutions and thawing methods for cryopreservation of embryos of mice and rats. T. N. Igonina, E. Yu. Brusentsev, I. N. Rozhkova, V. A. Naprimerov, S. Ya. Amstislavsky
Abstract: The proper choice of cryoprotectant and thawing method affects cryopreservation efficiency. A freezing-thawing method for sparing embryonic cells was evaluated in experiments with ICR mice. Cleavage-stage embryos of ICR mice, GC rats, and OXYS rats were collected on Day 3 …
Effects of growth factors during in vitro culture of mouse and rat embryos. E. Yu. Brusentsev, T. N. Igonina, I. N. Rozhkova, D. S. Ragaeva, S. Ya. Amstislavsky
Abstract: In vitro culture of preimplantation embryos of ICR, HT1AN/Icgn, HT1AC/Icgn and C57BL/6J-Ay mouse strains as well as in OXYS/Icgn rat strain in media containing granulocyte-macrophage colony stimulating factor (GM-CSF) or epidermal growth factor (EGF) has been studied. Both mouse and …
Applying reproductive technologies and genome resource banking to laboratory animals. S. Ya. Amstislavsky, E. Yu. Brusentsev, T. O. Abramova, D. S. Ragaeva, I. N. Rozhkova, T. N. Igonina, E. A. Kizilova, V. A. Naprimerov, N. Yu. Feoktistoiva
Abstract: The Genome Resource Bank (GRB) is a repository of frozen biological material, including semen and embryos. Cryobanking is used in combination with modern reproductive technologies such as rederivation, in vitro culture and embryo transfer. Thirteen mouse and rat strains …
